• Curriculum Vitae

Dr. Dali Li received his Ph. D degree from the joint program between Hunan Normal University and Texas A&M University Health Science Center in 2007. After graduation, he worked in School of Life Sciences, East China Normal University, and received promotion to Associate Professor in 2009. He has set up and directs the transgenic core facility in ECNU, and has published more than 20 articles in high-profile journals. His research is supported by National Natural Science Foundation, ECNU, State Key Development Programs of China (973).

• Research Interests

1) GPCRs in reproductive system: Hypothalamus-Pituitary-Gonads axis (HPG axis) plays a central role in puberty and life cycle. In the HPG axis, GPCRs (KISS1R, GnRHR, LHR, FSHR…) mediated signaling is the fundamental molecular basis and plays essential roles. We have focused our attention on the roles of GPCRs in somatic cells in testes and ovaries.

2) Using intestinal epithelium as a model to understand the roles of GPCR signaling in adult stem cell regulation, tissue regeneration and cancer. The intestinal epithelium is rapidly renewing and turns over every 3–5 days, which makes it an ideal model for tissue stem cell and cancer research. We generate knockout mouse models for distinct GPCRs and dissect the functions of these receptors in intestinal homeostasis and cancer.

• Publications in 2014:

1、Shao Y, Guan Y, Wang L, Qiu Z, Liu M, Chen Y, Wu L, Li Y, Ma X, Liu M*, Li D*. Genome editing in the rat using CRISPR-Cas and injection of RNAs into one-cell embryos. Nature Protocols. (In Press)

2、Guan X, Duan Y, Zeng Q, Pan H, Qian Y, Li D*, Cao X*, Liu M*. Lgr4 Deficiency Induces Ataxia-like Phenotype in Mice and Impairs Long-term Depression at Cerebellar Parallel fiber-Purkinje Cell Synapses. J Biol Chem. 2014 Jul 25. pii: jbc.M114.564138.

3、Pan H, Cui H, Liu S, Qian Y, Wu H, Li L, Guan Y, Guan X, Zhang L, Fan HY, Ma Y, Li R, Liu M*, Li D*. Lgr4 gene regulates Corpus Luteum Maturation through Modulation of the WNT mediated EGFR-ERK Signaling Pathway. Endocrinology. 2014 May 30:en20132183.

• Publications in 2013:

    1、Li D*, Qiu Z, Shao Y, Chen Y, Guan Y, Liu M, Li Y, Gao N, Wang L, Lu X, Zhao Y*, Liu M*. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nature Biotechnol. 2013 ;31(8):681-3.

    2、Luo W, Rodriguez M, Valdez JM, Zhu X, Tan K, Li D, Siwko S, Xin L, Liu M. Lgr4 is a Key Regulator of Prostate Development and Prostate Stem Cell Differentiation. Stem Cells. 2013;31(11):2492-505.

   3、Wang Y, Dong J, Li D, Lai L, Siwko S, Li Y, Liu M. Lgr4 regulates mammary gland development and stem cell activity through the pluripotency transcription factor Sox2. Stem Cells. 2013;31(9):1921-31.

    4、Qiu Z, Liu M, Chen Z, Shao Y, Pan H, Wei G, Yu C, Zhang L, Li X, Wang P, Fan HY, Du B, Liu B, Liu M, Li D*. High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases. Nucleic Acids Res. 2013 Jun;41(11):e120.

    5、Liu S, Qian Y, Li L, Wei G, Guan Y, Pan H, Guan X, Zhang L, Lu X, Zhao Y, Liu M*, Li D*.Lgr4 deficiency increases susceptibility and severity of dextran sodium sulphate induced inflammatory bowel disease in mice. J Biol Chem. 2013 288: 8794-8803. [paper of the week]

    6、Qian Y, Liu S, Guan Y, Pan H, Guan X, Qiu Z, Li L, Gao N, Zhao Y, Li X, Lu Y, Liu M*, Li D.* Lgr4 mediated Wnt/β-catenin signaling in peritubular myoid cells is essential for spermatogenesis.  Development. 2013;140(8):1751-61.

    7、Du B, Luo W, Li R, Tan B, Han H, Lu X, Li D, Qian M, Zhang D, Zhao Y, Liu M. Lgr4/Gpr48 negatively regulates TLR2/4-associated pattern recognition and innate immunity by targeting CD14 expression. J Biol Chem. 2013; 288(21):15131-41.

• other Publications :

    1、Cho SG, Li D, Tan K, Siwko SK, Liu M. KiSS1 and its G-protein-coupled receptor GPR54 in cancer development and metastasis. Cancer Metastasis Rev. 2012；31(3-4):585-91.

    2、Dai F, Chen Y, Song Y, Huang L, Zhai D, Dong Y, Lai L, Zhang T, Li D, Pang X, Liu M, Yi Z. A natural small molecule harmine inhibits angiogenesis and suppresses tumour growth through activation of p53 in endothelial cells. PLoS One. 2012;7(12):e52162

   3、Cho SG, Wang Y, Rodriguez M, Tan K, Zhang W, Luo J, Li D, Liu M Haploinsufficiency in the prometastasis Kiss1 receptor Gpr54 delays breast tumor initiation, progression, and lung metastasis. Cancer Res. 2011 15;71(20):6535-46.

    4、Yu W, Qiu Z, Gao N, Wang L, Cui H, Qian Y, Jiang L, Luo J, Yi Z, Lu H, Li D*, and Liu M* PAK1IP1, a ribosomal stress-induced nucleolar protein, regulates cell proliferation via the p53-MDM2 loop,Nucleic Acids Res 2010 39(6):2234-48.

    5、Dong Y, Lu B, Zhang X, Zhang J, Lai L, Li D, Wu Y, Song Y, Luo J, Pang X, Yi Z, Liu M. Cucurbitacin E, a Tetracyclic Triterpenes Compound from Chinese Medicine, Inhibits Tumor Angiogenesis through VEGFR2 Mediated Jak2/STAT3 Signaling Pathway. Carcinogenesis. 2010 Aug 23

    6、Yang Z, Li C, Wang X, Zhai C, Yi Z, Wang L, Liu B, Du B, Wu H, Guo X, Liu M, Li D*, Luo J*. Dauricine induces apoptosis, inhibits proliferation and invasion through inhibiting NF-kappaB signaling pathway in colon cancer cells. J Cell Physiol. 2010 Oct;225(1):266-75.

    7、Yi, T., Tan, K., Cho, S.G., Wang, Y., Luo, J., Zhang, W., Li, D. and Liu, M. Regulation of embryonic kidney branching morphogenesis and glomerular development by KISS1 receptor (GPR54) through NFAT2 and SP1 mediated bmp7 expression. J Biol Chem. 285(23):17811-20

    8、Li, X.Y., Lu, Y., Sun, H.Y., Wang, J.Q., Yang, J., Zhang, H.J., Fan, N.G., Xu, J., Jiang, J.J., Liu, R.Y., Li,D. et al. (2010) G protein-coupled receptor 48 upregulates estrogen receptor alpha expression via cAMP/PKA signaling in the male reproductive tract. Development, 137, 151-157.

    9、Li, C., Yang, Z., Zhai, C., Qiu, W., Li, D., Yi, Z., Wang, L., Tang, J., Qian, M., Luo, J. et al.Maslinic acid potentiates the anti-tumor activity of tumor necrosis factor alpha by inhibiting NF-kappaB signaling pathway. Mol Cancer, 9, 73

    10、Yi, Z.F., Cho, S.G., Zhao, H., Wu, Y.Y., Luo, J., Li, D., Yi, T., Xu, X., Wu, Z. and Liu, M. (2009) A novel peptide from human apolipoprotein(a) inhibits angiogenesis and tumor growth by targeting c-Src phosphorylation in VEGF-induced human umbilical endothelial cells. Int J Cancer, 124, 843-852.

    11、Wang, L., Kuang, L., Pan, X., Liu, J., Wang, Q., Du, B., Li, D., Luo, J., Liu, M., Hou, A. et al.(2010) Isoalvaxanthone inhibits colon cancer cell proliferation, migration and invasion through inactivating Rac1 and AP-1. Int J Cancer. 127(5):1220-9

    12、Luo, J., Zhou, W., Zhou, X., Li, D., Weng, J., Yi, Z., Cho, S.G., Li, C., Yi, T., Wu, X. et al.(2009) Regulation of bone formation and remodeling by G-protein-coupled receptor 48. Development, 136, 2747-2756.

    13、Li, D., Yu, W. and Liu, M. (2009) Regulation of KiSS1 gene expression. Peptides, 30, 130-138.

    14、Li, D*., Niu, Z., Yu, W., Qian, Y., Wang, Q., Li, Q., Yi, Z., Luo, J., Wu, X., Wang, Y. et al.(2009) SMYD1, the myogenic activator, is a direct target of serum response factor and myogenin. Nucleic Acids Res, 37, 7059-7071 .

    15、Cho, S.G., Yi, Z., Pang, X., Yi, T., Wang, Y., Luo, J., Wu, Z., Li, D. and Liu, M. (2009) Kisspeptin-10, a KISS1-derived decapeptide, inhibits tumor angiogenesis by suppressing Sp1-mediated VEGF expression and FAK/Rho GTPase activation. Cancer Res, 69, 7062-7070.

    16、Cho, S.G., Li, D., Stafford, L.J., Luo, J., Rodriguez-Villanueva, M., Wang, Y. and Liu, M. (2009) KiSS1 suppresses TNFalpha-induced breast cancer cell invasion via an inhibition of RhoA-mediated NF-kappaB activation. J Cell Biochem, 107, 1139-1149.

    17、Yu, W., Li, Y., Zhou, X., Deng, Y., Wang, Z., Yuan, W., Li, D., Zhu, C., Zhao, X., Mo, X. et al.(2008) A novel human BTB-kelch protein KLHL31, strongly expressed in muscle and heart, inhibits transcriptional activities of TRE and SRE. Mol Cells, 26, 443-453.

    18、Weng, J., Luo, J., Cheng, X., Jin, C., Zhou, X., Qu, J., Tu, L., Ai, D., Li, D., Wang, J. et al.(2008) Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2. Proc Natl Acad Sci U S A, 105, 6081-6086.

    19、Song, H., Luo, J., Luo, W., Weng, J., Wang, Z., Li, B., Li, D. and Liu, M. (2008) Inactivation of G-protein-coupled receptor 48 (Gpr48/Lgr4) impairs definitive erythropoiesis at midgestation through down-regulation of the ATF4 signaling pathway. J Biol Chem, 283, 36687-36697.

    20、Ma, W., Stafford, L.J., Li, D., Luo, J., Li, X., Ning, G. and Liu, M. (2007) GCIP/CCNDBP1, a helix-loop-helix protein, suppresses tumorigenesis. J Cell Biochem, 100, 1376-1386.

    21、Li, D., Mitchell, D., Luo, J., Yi, Z., Cho, S.G., Guo, J., Li, X., Ning, G., Wu, X. and Liu, M. (2007) Estrogen regulates KiSS1 gene expression through estrogen receptor alpha and SP protein complexes. Endocrinology, 148, 4821-4828.

   22、Mitchell DC, Stafford LJ, Li D, Bar-Eli M, Liu M. (2007) Transcriptional regulation of KiSS-1 gene expression in metastatic melanoma by specificity protein-1 and its coactivator DRIP-130. Oncogene. 26(12):1739-47